opentrons-integration
Automate lab workflows with Opentrons robots
Also available from: davila7
Writing lab automation protocols requires specialized knowledge of robotics APIs and liquid handling procedures. This skill provides ready-to-use templates and comprehensive guidance for creating protocols that control Opentrons liquid handling robots.
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Using "opentrons-integration". Create a protocol for plating bacterial cultures from a 12-well reservoir to a 96-well plate
Expected outcome:
- Generated complete Opentrons protocol with:
- - P300 single-channel pipette on left mount
- - 12-well reservoir in deck slot D2
- - 96-well plate in slot D3
- - 200µL tip rack in slot D1
- - 50µL transfer from each reservoir well to plate columns
- - Automatic tip changes between samples
Using "opentrons-integration". Write a protocol for ELISA sample preparation across 8 rows
Expected outcome:
- Created ELISA prep protocol:
- - Loaded 8 tip racks and 2 reagent reservoirs
- - P300 multi-channel pipette for row-wise operations
- - Added 100µL coating antibody to all 96 wells
- - Performed 3 wash steps between incubations
- - Included 5-minute incubation delays
Security Audit
SafeAll 360 static findings are false positives. The analyzer misinterpreted markdown backticks as shell commands, API version strings as weak crypto, and lab equipment terms as reconnaissance. This is a legitimate lab automation skill containing Opentrons protocol templates for liquid handling robots.
Risk Factors
⚙️ External commands (1)
Quality Score
What You Can Build
Automate PCR setup
Create protocols for setting up PCR reactions with master mix distribution and thermocycler programming
Perform serial dilutions
Generate accurate serial dilution protocols across 96-well plates for assay development
Control multiple modules
Integrate temperature, magnetic, and heater-shaker modules in complex workflows
Try These Prompts
Write an Opentrons protocol that transfers 100µL from well A1 to B1 using a P300 pipette
Create a protocol for setting up 24 PCR reactions with master mix and DNA samples using the thermocycler
Write a protocol for 1:2 serial dilutions across a 96-well plate row, starting with 200µL stock
Create a protocol using temperature module for sample incubation at 37°C followed by magnetic bead separation
Best Practices
- Always simulate protocols before running on actual hardware
- Use meaningful labels for labware to simplify troubleshooting
- Include protocol comments for complex steps and timing requirements
Avoid
- Hardcoding absolute positions instead of using well references
- Mixing different API levels within the same protocol
- Forgetting to account for tip consumption in long protocols